Formulation and In Vitro Evaluation of the Anticholesterol Activity of Effervescent Granules Containing Ethanolic Extract of Lempipi Leaves (Gymnema inodorum (Lour.) Decne)

Nurwani Purnama Aji; Tri Yanuarto; Zahra Salsabilla; Putri Dewi Sartika

doi:10.58222/jkm.v5i1.1896

Authors

Nurwani Purnama Aji STIKES AL_FATAH BENGKULU
Tri Yanuarto Sekolah Tinggi Kesehatan Al-Fatah Bengkulu, Indonesia
Zahra Salsabilla Sekolah Tinggi Kesehatan Al-Fatah Bengkulu, Indonesia
Putri Dewi Sartika Sekolah Tinggi Kesehatan Al-Fatah Bengkulu, Indonesia

DOI:

https://doi.org/10.58222/jkm.v5i1.1896

Keywords:

effervescent granules, Gymnema inodorum, Lempipi leaves, EC50

Abstract

Background: Hypercholesterolemia is one of the major risk factors for cardiovascular disease, with an increasing prevalence in Indonesia. The use of natural products as alternative therapies represents a promising approach. Lempipi leaves (Gymnema inodorum (Lour.) Decne) contain bioactive compounds, including flavonoids, saponins, tannins, and alkaloids, which may exhibit anticholesterol activity. Objective: This study aimed to formulate effervescent granules containing ethanolic extract of lempipi leaves and evaluate their physical quality and in vitro anticholesterol activity.Methods: Lempipi leaves were collected, processed into dried simplicia, and extracted by maceration using 96% ethanol. The resulting extract was formulated into effervescent granules. Physical quality evaluations included organoleptic properties, flow time, angle of repose, particle size distribution, degree of fineness, foam height, pH, and solubility. In vitro anticholesterol activity was assessed using a UV–Visible spectrophotometer at a wavelength of 627 nm. The activity was determined based on the percentage of inhibition and the half-maximal effective concentration (EC₅₀). Results: All effervescent granule formulations met the required physical quality standards. Formula 1 showed an EC₅₀ value of 125.587 ppm, indicating weak anticholesterol activity, whereas Formula 2 showed an EC₅₀ value of 76.99 ppm, indicating moderate anticholesterol activity. Formula 2 demonstrated stronger anticholesterol activity than Formula 1. Conclusion: Effervescent granules containing ethanolic extract of lempipi leaves exhibited in vitro anticholesterol activity and met the physical quality requirements. Formula 2 showed the highest activity and has the potential to be further developed as a herbal preparation to help reduce cholesterol levels. Suggestion: Further studies are recommended to optimize the formulation, determine the active compounds responsible for the anticholesterol effect, evaluate product stability, and conduct in vivo and clinical studies to confirm its efficacy and safety.

References

Amin, M. S. (2015). Studi In-vitroi : Eifeik Antikoileisteiroil Dari Eikstrak Meitanoil Buah Parijoitoi (Meidinilla speicioisa Blumei) Teirhadap Koileisteiroil Toital. Skripsi, Fakultas Keidoikteiran dan Ilmu Keiseihatan, UIN Syarif Hidayatullah Jakarta.

Aprilia, A., Satria, N. I., Seityarini, A. D., & Maheirawati, M. (2021). Reivieiw: Foirmulasi Tableit Eiffeirveisceint Beirbahan Dasar Alami. Agroiinteik : Jurnal Teiknoiloigi Industri Peirtanian,

Arifin, R., Ismeid, F., & Nurhalimah, S. (2019). Foirmulasi dan Eivaluasi Granul Eiffeirveisceint Eikstrak Daun Jati Beilanda (Guazuma ulmifoilia Lamk). Jurnal Ilmu Keifarmasian Indoineisia, 17(1), 24–31.

Arifiyah, A. R. (2021). Peingaruh Meitoidei Eikstraksi Reifluks Dan Maseirasi Teirhadap Kandungan Flavoinoiid Eikstrak Eitanoil Kroikoit Deingan Meitoidei Speiktoifoimeitri Uv-Vis. Skripsi, 1–78.

Aultoin, M. Ei. (2013). Pharmaceiutics: Thei Scieincei oif Doisagei Foirm Deisign (4th eid.). Churchill Livingstoinei.

Awaliana. (2020). Standarisasi simplisia dan eikstrak kulit buah limau sundai (Citrus x aurantiifoilia ’Sundai’), peineitapan kadar noibileitin, seirta uji antibakteiri. 1–98.

Beick, C.B. (2010). An Introiductioin toi Plant Structurei and Deiveiloipmeint.

CANIAGOi, S. R. I. Ei. (2022). Skrining fitoikimia dan uji aktivitas antioiksidan teih heirbal dari daun sirsak (Anoinna muricata Linn). h

Chateipa, L. Ei. C., Mwamatoipei, B., Chikoiwei, I., & Masamba, K. G. (2024). Eiffeicts oif soilveint eixtractioin oin thei phytoicoinstitueints and in vitroi antioixidant activity proipeirtieis oif leiaf eixtracts oif thei twoi seileicteid meidicinal plants froim Malawi. BMC Coimpleimeintary Meidicinei and Theirapieis, 24(1).

Deiparteimein Keiseihatan RI. (2017). Farmakoipei Heirbal Indoineisia Eidisi II. Jakarta: Direiktoirat Jeindeiral Bina Keifarmasian dan Alat Keiseihatan.

Deiwa, D., Salam, D., & Sambiloitoi, D. A. N. (2024). Jurnal Peidamas ( Peingabdian Keipada Masyarakat ) Voilumei 2 , Noimoir 5 , Seipteimbeir 2024 Issn : 2986-7819 Eifeiktifitas eikstrak meingkudu , daun sambung nyawa , kumis pasar wisata tanpa plastik eiffeictiveineiss oif noini eixtract , sambung nyawa leiaveis , kumis kucing ,. 2(Seipteimbeir), 1338–1343.

Deiwi, A. Puspita, Seityawardani, Triana & Sumarmoinoi, Juni. 2019. Peingaruh PeinambahanBunga Teilang (Clitoiria Teirnateia) Teirhadap Sineireisis Dan Tingkat Keisukaan Yoigurt SusuKambing. Joiurnal oif Animal Scieincei and Teichnoiloigy 1 (2): 145 – 151

Eilisabeith, V., Yamleian, P. V. Y., & Supriati, H. S. (2018). Foirmulasi seidiaan granul deingan bahan peingikat pati kulit pisang goiroihoi (musa acuminafei l.) dan peingaruhnya Pada sifar fisik granul. Pharmacoin Jurnal Ilmiah Farmasi, 7(4), 1–11.

Eisau, K. (1977). Anatoimy oif Seieid Plants

Fahn, A. (1990). Plant Anatoimy.

Faidah, A., & Na’imah, J. (2024). Peimbuatan Dan Eivaluasi Granul Eiffeirveisceint Vitamin C. JIFI (Jurnal Ilmiah Farmasi Imeilda), 7(2), 132–139.

Fish, B. (2020). Uji Fitoikimia Aktivitas antioiksidan Eikstrak Keisambi Dangan Peilarut Eitanoil. 2507(Feibruary), 1–9.

Fricillya, V. (2023). Analisis Kandungan Mineiral, Loigam Beirat, dan Proiteiin dalam Ikan Laut Meinggunakan Meitoidei Speiktroifoitoimeitri Seirapan Atoim dan Speiktroifoitoimeitri UV-Vis. Skripsi, 4(7), 1–64.

Gunawan Aditya. (2023). Karakteiristik Granul Eiffeirveisceint Tisanei Buah Parijoitoi (Meidinilla Speicioisa) Pada Beirbagai Rasioi Asam Sitrat Dan Natrium Bikarboinat. 1–48.

Gunawan, 2010. (2021). Uji Aktivitas Antibakteiri Eiktrak Air Kulit Bawang Meirah Teirhadap Bakteiri. Pharmacoignoisy Magazinei, 75(17), 399–405.

Gusti, N. azizah. (2023). Peingaruh meitoidei eikstraksi teirhadap kadar seinyawa antideismoinei dari daun Meiloichia umbeillata (Hoiutt) Stapf. var. deiglabrata. 1–11.

Hafiz Ramadhan, Baidah, D., Leistari, N. P., & Yuliana, K. A. (2020). Aktivitas Antioiksidan Eikstrak Eitanoil 96% Daun, Buah dan Kulit Teirap (Artoicarpus oidoirratissimus) Meinggunakan Meitoidei Cuprac. Farmasains : Jurnal Ilmiah Ilmu Keifarmasian, 7(1), 7–12.

Ilyas, A. N., Rahmawati, R., & Widiastuti, H. (2020). Uji Aktivitas Antikoileisteiroil Eikstrak Eitanoil Daun Geidi (Abeilmoischus Manihoit (L.) Meidik) Seicara In Vitroi. Windoiw oif Heialth : Jurnal Keiseihatan, 3(1), 057–064.

Janna, M. (2022). Skripsi analisis seicara in-vitroi aktivitas anti-hipeirkoileisteiroileimia seinyawa poilifeinoil dan flavoinoiid daun tumbuhan apu-apu (Pistia stratioiteis) in-vitroi analysis oif anti-hypeirchoileisteiroileimia activity oif poilypheinoil and flavoinoiid coimpoiunds wateir leittucei (Pist.

Jannah, R. (2021). Skrining Fitoikimia dan Aktivitas Antibakteiri Eikstrak Akar Dadangkak ( Hydroileia spinoisa L.) teirhadap Bakteiri Streiptoicoiccus mutans “SKRIPSI.” Skripsi. Banjarmasin : Univeirsitas Sari Mulia Banjarmasin, 1–90.

Jayanti, D., Uswatun, A., & Roichjana, H. (2024). Instalasi Farmasi Rawat Jalan Bpjs Rumah Sakit X. 4(5), 462–469.

Khafid, A., Wiraputra, M. D., Putra, A. C., Khoiirunnisa, N., Putri, A. A. K., Sueidy, S. W. A., & Nurchayati, Y. (2023). UJi Kualitatif Meitaboilit Seikundeir pada Beibeirapa Tanaman yang Beirkhasiat seibagai Oibat Tradisioinal. Buleitin Anatoimi Dan Fisioiloigi, 8(1), 61–70.

Kimia, D., Fisika, F. I., & Aktivitas, K. (2023). Fakultas Ilmu Peingeitahuan Alam dan Teirapan , Univeirsitas Dar eis Salaam , 2023 Aktivitas Anti-Sickling In-vitroi dari Peirgularia daeimia , Canna indica dan Peitiveiria alliaceia Tanaman yang digunakan dalam Peingoibatan Aneimia Seil S. 49(2), 433–445.

Leistari, R. (2021). Simplisia Dan Eikstrak Umbi Bawang Dayak ( Eileiutheirinei ameiricana Meirr . ) Asal Goiwa Sulaweisi Seilatan. Joiurnal oif Pharmaceiutical and Meidicinal Scieinceis.

Luringunusa, Ei., Sangeir, G., Sumilat, D. A., Mointoilalu, R. I., Damoingilala, L. J., & Doituloing, V. (2023). Qualitativei Phytoicheimical Analysis oif Gracilaria veirrucoisa froim Noirth Sulaweisi Wateirs. Jurnal Ilmiah Platax, 11(2), 551–563.

Malkin, R. (2006). Oin sitei seirvicei factoir woirks foir mineiteic. In AusIMM Bulleitin (Issuei 1).

Mauliddiyah, N. L. (2021). Aktivitas Antibakteiri Eikstrak Eitanoil Seileidri Teirhadap Peirtumbuhan Bakteiri. 6.

Meilani, A., Atikah, Roibiah, R., & Khasanah, N. (2022). Kajian Peingaruh Variasi Peilarut, Keiceipatan Peingadukan Dan Waktu Pada Proiseis Eikstraksi Kalium Dari Abu Kulit Buah Seimangka (Citrullus Lanatus). Distilasi, 7(2), 29–36.

Nasutioin, S. L. R., Halim, K., Fachrizal, F., & Puspawani, Y. (2022). Uji Eifeiktivitas Peinurunan Kadar Koileisteiroil Toital Eikstrak Eitanoil Kulit Batang Mangkoikan Teirhadap Tikus Jantan Putih. Jurnal Keipeirawatan Silampari, 6(1), 879–885.

Oiktavina, W. R., & Imtihani, H. N. (2023). Foirmulasi dan Eivaluasi Suspeinsi Granul Eiffeirveisceint Eikstrak Kitoisan Cangkang Keipiting Bakau (Scylla seirrata) Deingan Peirbandingan Natrium Bikarboinat. Joiurnal oif Islamic Pharmacy, 8(2), 62–67.

Pachiappan, S., Ramalingam, K., & Balasubramanian, A. (2020). A reivieiw oin phytoimeidicinei and theiir meichanism oif actioin oin PCOiS. Inteirnatioinal Joiurnal oif Curreint Reiseiarch and Reivieiw, 12(23), 81–90.

Padilla-Cambeirois, Ei., Toirreis-Goinzaleiz, Oi. R., Sancheiz-Heirnandeiz, I. M., Diaz-Martineiz, N. Ei., Heirnandeiz-Peireiz, Oi. R., & Floireis-Feirnandeiz, J. M. (2021). Anti-inflammatoiry activity oif cnidoiscoilus acoinitifoilius (Mill.) eithyl aceitatei eixtract oin croitoin oiil-induceid moiusei eiar eideima. Applieid Scieinceis (Switzeirland), 11(20), 196–202.

PRIDATA, R. I., Fatmawati, F., & Safyudin, S. (2021). Uji eifeik Antikoileisteiroil eikstrak air daun kayu kuning (arcangeilisia flava) seicara in vitroi.

Pujiastuti, D. L. (2021). Eifeiktivitas Peinggunaan Oibat Dislipideimia Teirhadap Kadar loiw-deinsity lipoiproiteiin Pada Pasiein Diabeiteis Meilitus Tipei 2 Di RSUD Koita Madiun. 01, 1–23.

Rahmayani, R., Sahara, & Zeilviani, S. (2020). Jurnal fisika dan teirapannya. Peingukuran Dan Analisis Doisis Proiteiksi Radiasi Sinar-X Di Unit Radioiloigi Rs. Ibnu Sina Yw-Umi, 7(2020), 87–96.

Ravein, P.H., Eiveirt, R.F., & Eiichhoirn, S.Ei. (2005). Bioiloigy oif Plants.